- Crab duplex-specific nuclease
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Crab duplex-specific nuclease is a nuclease derived from kamchatka crab hepatopancreas that displays a strong preference for cleaving double-stranded DNA and DNA in DNA–RNA hybrid duplexes, compared to single-stranded DNA. Moreover, the cleavage rate of short, perfectly matched DNA duplexes by this enzyme is essentially higher than that for non-perfectly matched duplexes of the same length. It has been applied to SNP detection[1] and RNA normalization.[2]
References
- ^ Shagin, DA; Rebrikov, DV, Kozhemyako, VB, Altshuler, IM, Shcheglov, AS, Zhulidov, PA, Bogdanova, EA, Staroverov, DB, Rasskazov, VA, Lukyanov, S (2002 Dec). "A novel method for SNP detection using a new duplex-specific nuclease from crab hepatopancreas". Genome research 12 (12): 1935–42. doi:10.1101/gr.547002. PMC 187582. PMID 12466298. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=187582.
- ^ Christodoulou, DC; Gorham, JM, Herman, DS, Seidman, JG (2011 Apr). "Construction of Normalized RNA-seq Libraries for Next-Generation Sequencing Using the Crab Duplex-Specific Nuclease". Current Protocols in Molecular Biology Chapter 4: Unit 4.12. PMID 21472699.
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