Immunomagnetic separation
- Immunomagnetic separation
Immunomagnetic separation (IMS) is a laboratory tool that can efficiently isolate cells out of body fluid or cultured cells. It can also be used as a method of quantifying the pathogenicity of food, blood or feces. DNA analysis have supported the combined use of both this technique and Polymerase Chain Reaction (PCR).
Technique
Antibodies coating paramagnetic beads will bind to antigens present on the surface of cells thus "capturing" the cells and facilitate the concentration of these bead-attached cells. The concentration process is created by a magnet placed on the side of the test tube bringing the beads to it.
ource
#Engstrand, L. and Enroth, H., "Journal of Clinical microbiology", vol.33, no.8, August 1995, p. 2162-2165.
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