- Immunophenotyping
Immunophenotyping is a technique used to study the protein expressed by cells. This technique is commonly used in basic science research and laboratory diagnostic purpose. This can be done on tissue section (fresh or fixed tissue), cell suspension, etc. An example is the
diagnosis ofleukemia . It involves the labelling ofwhite blood cell s with antibodies directed against surfaceprotein s on their membrane. By choosing appropriate antibodies, the differentiation of leukemic cells can be accurately determined. The labelled cells are processed in a flow cytometer, a laser-based instrument capable of analyzing thousands of cells per second. The whole procedure can be performed on cells from theblood ,bone marrow orspinal fluid in a matter of a few hours.An Example of Information provided through Immnunophenotyping"The flow cytometric immunophenotyping report indicated the malignant cells were positive for CD19, CD10, dimCD20, CD45, HLA-DR, and λ immunoglobulin light chain. There was no coexpression of CD5 or CD23 by the monoclonal
B-cell population."External links
* [http://www.bcshguidelines.com/pdf/clh190.pdf British Society for Haematology guidelines] accessed July 31, 2006
* [http://pleiad.umdnj.edu/hemepath/immuno/immuno.html "Immunophenotyping Lymphomas", University of Medicine & Dentistry of New Jersey]
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