DFFA

DFFA
DNA fragmentation factor, 45kDa, alpha polypeptide

PDB rendering based on 1iyr.
Identifiers
Symbols DFFA; DFF-45; DFF1; ICAD
External IDs OMIM601882 MGI1196227 HomoloGene3240 GeneCards: DFFA Gene
RNA expression pattern
PBB GE DFFA 203277 at tn.png
More reference expression data
Orthologs
Species Human Mouse
Entrez 1676 13347
Ensembl ENSG00000160049 ENSMUSG00000028974
UniProt O00273 Q8BQC7
RefSeq (mRNA) NM_004401.2 NM_001025296
RefSeq (protein) NP_004392.1 NP_001020467
Location (UCSC) Chr 1:
10.52 – 10.53 Mb
Chr 4:
148.48 – 148.49 Mb
PubMed search [1] [2]
DNA Fragmentation factor 45kDa, C terminal domain
PDB 1koy EBI.jpg
nmr structure of dff-c domain
Identifiers
Symbol DFF-C
Pfam PF09033
InterPro IPR015121

DNA fragmentation factor subunit alpha is a protein that in humans is encoded by the DFFA gene.[1][2][3]

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[3]

The C-terminal domain of DFFA (DFF-C) consists of four alpha-helices, which are folded in a helix-packing arrangement, with alpha-2 and alpha-3 packing against a long C-terminal helix (alpha-4). The main function of this domain is the inhibition of DFFB by binding to its C-terminal catalytic domain through ionic interactions, thereby inhibiting the fragmentation of DNA in the apoptotic process. In addition to blocking the DNase activity of DFFB, the C-terminal region of DFFA is also important for the DFFB-specific folding chaperone activity, as demonstrated by the ability of DFFA to refold DFFB.[4]

Interactions

DFFA has been shown to interact with DFFB.[5][6]

References

  1. ^ Leek JP, Carr IM, Bell SM, Markham AF, Lench NJ (Jun 1998). "Assignment of the DNA fragmentation factor gene (DFFA) to human chromosome bands 1p36.3→p36.2 by in situ hybridization". Cytogenet Cell Genet 79 (3–4): 212–3. doi:10.1159/000134725. PMID 9605855. 
  2. ^ Liu X, Zou H, Slaughter C, Wang X (May 1997). "DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis". Cell 89 (2): 175–84. doi:10.1016/S0092-8674(00)80197-X. PMID 9108473. 
  3. ^ a b "Entrez Gene: DFFA DNA fragmentation factor, 45kDa, alpha polypeptide". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=1676. 
  4. ^ Fukushima K, Kikuchi J, Koshiba S, Kigawa T, Kuroda Y, Yokoyama S (August 2002). "Solution structure of the DFF-C domain of DFF45/ICAD. A structural basis for the regulation of apoptotic DNA fragmentation". J. Mol. Biol. 321 (2): 317–27. doi:10.1016/S0022-2836(02)00588-0. PMID 12144788. 
  5. ^ Ewing, Rob M; Chu Peter, Elisma Fred, Li Hongyan, Taylor Paul, Climie Shane, McBroom-Cerajewski Linda, Robinson Mark D, O'Connor Liam, Li Michael, Taylor Rod, Dharsee Moyez, Ho Yuen, Heilbut Adrian, Moore Lynda, Zhang Shudong, Ornatsky Olga, Bukhman Yury V, Ethier Martin, Sheng Yinglun, Vasilescu Julian, Abu-Farha Mohamed, Lambert Jean-Philippe, Duewel Henry S, Stewart Ian I, Kuehl Bonnie, Hogue Kelly, Colwill Karen, Gladwish Katharine, Muskat Brenda, Kinach Robert, Adams Sally-Lin, Moran Michael F, Morin Gregg B, Topaloglou Thodoros, Figeys Daniel (2007). "Large-scale mapping of human protein–protein interactions by mass spectrometry". Mol. Syst. Biol. (England) 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1847948. 
  6. ^ McCarty, J S; Toh S Y, Li P (Oct. 1999). "Study of DFF45 in its role of chaperone and inhibitor: two independent inhibitory domains of DFF40 nuclease activity". Biochem. Biophys. Res. Commun. (UNITED STATES) 264 (1): 176–80. doi:10.1006/bbrc.1999.1497. ISSN 0006-291X. PMID 10527860. 

Further reading



This article includes text from the public domain Pfam and InterPro IPR015121


Wikimedia Foundation. 2010.

Игры ⚽ Поможем написать реферат

Look at other dictionaries:

  • DFFB — DNA fragmentation factor, 40kDa, beta polypeptide (caspase activated DNase) PDB rendering based on 1ibx …   Wikipedia

  • CIDEA — Cell death inducing DFFA like effector a, also known as CIDEA, is a human gene.cite web | title = Entrez Gene: CIDEA cell death inducing DFFA like effector a| url = http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene Cmd=ShowDetailView… …   Wikipedia

  • — I zhōu (1)  ㄓㄡˉ (2) 〔~啾〕形容鸟叫声。 (3) 〔~噍〕形容鸟叫声。 (4) 郑码: JLBJ, U: 5541, GBK: DFFA (5) 笔画数: 11, 部首: 口, 笔顺编号: 25135121251 II zhāo (1)  ㄓㄠˉ (2) 〔~哳〕形容声音杂乱细碎。 (3) 笔画数: 11, 部首: 口, 笔顺编号: 25135121251 (4) 郑码: JLBJ, U: 5541, GBK: DFFA (5) 笔画数: 11, 部首: 口,… …   International standard chinese characters dictionary

  • CIDEB — Recently, Cideb knock out mice have been generated by homolog recombination technique. The Cide null mice show decreased lipogenesis The Cideb knockout mice are resistant to high fat diet induced obesity and liver steatosis. In addition, the… …   Wikipedia

  • Catechin — IUPAC name (2R,3S) 2 (3,4 dihydroxyphenyl) 3,4 dihydro 2H chromene 3,5,7 …   Wikipedia

  • Raoul Peck — (b. 1953, Port au Prince, Haiti) is an award winning Haitian filmmaker, of both documentary and feature films, and a political activist. Briefly, in the 1990s, he was Haiti s Minister of Culture.BiographyAt age 8, the Haitan born Peck and his… …   Wikipedia

  • Stephen Clark — may refer to: * [(Stephen Clark (WXYZ TV)] formerly primary anchor WCBS TV, KGTV TV [http://www.wxyz.com/content/about7/bios/story.aspx?content id=9686c05a dffa 4cd7 aaef 1085f8be8f75] *Stephen Clark (NY treasurer), NYS Treasurer 1856 1857… …   Wikipedia

  • Feuerwehrsport — Dieser Artikel oder Absatz stellt die Situation in Deutschland dar. Hilf mit, die Situation in anderen Ländern zu schildern. Der Feuerwehrsport ist eine meist professionell betriebene Sportart, die aus vielen Bereichen der Feuerwehrarbeit heraus… …   Deutsch Wikipedia

Share the article and excerpts

Direct link
Do a right-click on the link above
and select “Copy Link”