Transfer gene

Transfer gene

Transfer operon, commonly called tra operon, or tra genes, are some of the genes necessary for non-sexual transfer of genetic material in both gram-positive and gram-negative bacteria. The tra locus includes the pilin gene and regulatory genes, which together form pili on the cell surface, polymeric proteins that can attach themselves to the surface of F- bacteria and initiate the conjugation. The existence of the tra region of a plasmid genome was first discovered in 1979 by David H. Figurski and Donald R. Helinskicite journal | author = Figurski D, Helinski D | title = Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function in "trans" | journal = PNAS | year = 1979 | volume = 76 | pages = 1648–1652 | url = http://www.pnas.org/cgi/content/abstract/76/4/1648 | doi = 10.1073/pnas.76.4.1648 ] In the course of their work, Figurski and Helinski also discovered a second key fact about the tra region - that it can act in "trans" to the mobilization marker which it affects.

This finding suggested that there were two basic aspects necessary for a plasmid to move from one cell to another:

1. An origin of transfer - The oriT (or mob) site must be located on the plasmid itself to allow recognition of the plasmid and initiation of transfer.cite journal | author = Fu Y H, Tsai M M, Luo Y N, Deonier R C | title = Deletion analysis of the F plasmid oriT locus. | journal = Journal of Bacteriology | year = 1991 | volume = 173(3) | pages = 1012–1020 | url = http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=207219 | pmid = 1991706 ] A plasmid with no origin of transfer is non-mobilizable.

2. The transfer genes - Though a functioning set of tra genes is necessary for plasmid transfer, they may be located in a variety of places including the plasmid in question, another plasmid in the same host cell, or even in the bacterial genome.cite journal | author = Simon R, Priefer U, Puhler A | title = A Broad Host Range Mobilization System for "In Vivo" Genetic Engineering: Transposon Mutagenesis in Gram Negative Bacteria | journal = Nature Biotechnology | year = 1983 | volume = 1 | pages = 784–791 | url = http://www.nature.com/nbt/journal/v1/n9/abs/nbt1183-784.html | doi = 10.1038/nbt1183-784 ]

The tra genes encode proteins which are useful for the propagation of the plasmid from the host cell to a compatible donor cell or maintenance of the plasmid. It is important to note that not all transfer operons are the same. Some genes are only found in a few species or a single genus of bacteria while others (such as "traL") are found in very similar forms in many bacterial species. Many of the transfer systems are incompatible. For example, "oriT" and "mob" are two origins of transfer which interact with different sets of transfer genes. A plasmid with a mob site (like many found in "Rhodococcus" species) cannot be transferred via transfer genes which normally interact with the oriT site (which is common in "E. coli")

Each of the individual genes in the tra operon codes for a different protein product. These products may perform a number of tasks including interaction with one another to perform mating pair functions and regulation of different regions of the tra operon itself,cite journal | author = Grohmann E, Muth G, Espinosa M | title = Conjugative Plasmid Transfer in Gram-Positive Bacteria | journal = Microbiology and Molecular Biology Reviews | year = 2003 | volume = 67(2) | pages = 277–301 | url = http://mmbr.asm.org/cgi/content/full/67/2/277 | doi = 10.1128/MMBR.67.2.277-301.2003 ] or conjugative DNA metabolism and surface exclusion. Also note that some proteins perform multiple functions or are associated closely with proteins which have non-similar functions.

References


Wikimedia Foundation. 2010.

Игры ⚽ Нужна курсовая?

Look at other dictionaries:

  • Gene sharing — This article is about genes sharing multiple functions in an organism; for gene sharing between organisms, see horizontal gene transfer. Gene sharing is when a single polypeptide sequence, encoded by a single gene, performs multiple distinct… …   Wikipedia

  • Gene therapy — using an Adenovirus vector. A new gene is inserted into an adenovirull. If the treatment is successful, the new gene will make a functional protein. Gene therapy is the insertion, alteration, or removal of genes within an individual s cells and… …   Wikipedia

  • Gene synthesis — is the process of synthesizing an artificially designed gene into a physical DNA sequence.Gene synthesis was first demonstrated by Har Gobind Khorana in 1970 for a short artificial gene. Nowadays, commercial gene synthesis services are available… …   Wikipedia

  • Gene Hunt — Life on Mars and Ashes to Ashes character Philip Glenister as Gene Hunt in Ashes to Ashes …   Wikipedia

  • Gene doping — is defined by the World Anti Doping Agency as the non therapeutic use of cells, genes, genetic elements, or of the modulation of gene expression, having the capacity to improve athletic performance . [http://www.wada… …   Wikipedia

  • gene transfer — gene′ trans fer n. gen lab the insertion of copies of a gene into living cells in order to induce synthesis of the gene s product …   From formal English to slang

  • Gene Morrison — Gene Anthony “Rocky” Morrison (born 1958) was a fraudulent forensic detective who operated over three decades in the Hyde area of Greater Manchester, England, under the title Dr. Gene Morrison. Memorable for his trademark sheepskin jacket; during …   Wikipedia

  • Gene flow — In population genetics, gene flow (also known as gene migration) is the transfer of alleles of genes from one population to another.Migration into or out of a population may be responsible for a marked change in allele frequencies (the proportion …   Wikipedia

  • Gene expression — For vocabulary, see Glossary of gene expression terms. For a non technical introduction to the topic, see Introduction to genetics. Genes are expressed by being transcribed into RNA, and this transcript may then be translated into protein. Gene… …   Wikipedia

  • Transfer RNA — tRNA ist die Kurzform für transfer RNA. Transfer RNAs sind kurze Ribonukleinsäuren (RNA). Die Länge reifer tRNAs liegt in der Regel zwischen 73 und 95 Nukleotiden. Sie vermitteln bei der Translation die richtige Aminosäure zum entsprechenden… …   Deutsch Wikipedia

Share the article and excerpts

Direct link
Do a right-click on the link above
and select “Copy Link”