Smart ligands

Smart ligands

Smart ligands are affinity ligands selected with pre-defined equilibrium (K_{d}), kinetic (k_{off}, k_{on}) and thermodynamic (ΔH, ΔS) parameters of biomolecular interaction.

Ligands with desired parameters can be selected from large combinatorial libraries of biopolymers using instrumental separation techniques with well-described kinetic behaviour, such as Kinetic capillary electrophoresis (KCE), Surface Plasmon Resonance (SPR) and etc. Known examples of smart ligands include DNA smart aptamers; however, RNA and peptide smart aptamers can also be developed.

Smart ligands can find a set of unique applications in biomedical research, drug discovery and proteomic studies. For example, a panel of DNA smart aptamers has been recently used to develop affinity analysis of proteins with ultra-wide dynamic range of measured concentrations.

References

* Drabovich, A.; Berezovski, M.; Krylov, S.N. Selection of smart aptamers by equilibrium capillary electrophoresis of equilibrium mixtures (ECEEM). "J. Am. Chem. Soc." 2005, 127, 11224-11225. [http://www.ncbi.nlm.nih.gov/sites/entrez?Db=PubMed&Cmd=ShowDetailView&TermToSearch=16089434 PMID: 16089434]
* Drabovich, A.P. ; Berezovski, M.; Okhonin, V.; Krylov, S.N. Selection of smart aptamers by methods of kinetic capillary electrophoresis. "Anal. Chem." 2006, 78, 3171-3178. [http://www.ncbi.nlm.nih.gov/sites/entrez?Db=PubMed&Cmd=ShowDetailView&TermToSearch=16643010 PMID: 16643010]
* Drabovich, A.P. ; Okhonin, V.; Berezovski, M.; Krylov, S.N. Smart aptamers facilitate multi-probe affinity analysis of proteins with ultra-wide dynamic range of measured concentrations. "J. Am. Chem. Soc." 2007, 129, 7260-7261. [http://www.ncbi.nlm.nih.gov/sites/entrez?Db=PubMed&Cmd=ShowDetailView&TermToSearch=17503828 PMID: 17503828]
* Krylov, S.N. Kinetic CE: foundation for homogeneous kinetic affinity methods. "Electrophoresis" 2007, 28, 69-88. [http://www.ncbi.nlm.nih.gov/sites/entrez?Db=PubMed&Cmd=ShowDetailView&TermToSearch=17245689 PMID: 17245689]

External links

* [http://pubs.acs.org/cen/news/85/i25/8525notw6.html Extending Protein Detection ]


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