Small nucleolar RNA SNORD95

snoRNA U95 (also known as SNORD95 or Z38) is a non-coding RNA (ncRNA) molecule which functions in the modification of other small nuclear RNAs (snRNAs). This type of modifiying RNA is usually located in the nucleolus of the eukaryotic cell which is a major site of snRNA biogenesis. It is known as a small nucleolar RNA (snoRNA) and also often referred to as a guide RNA.

snoRNA U95 belongs to the C/D box class of snoRNAs which contain the conserved sequence motifs known as the C box (UGAUGA) and the D box (CUGA). Most of the members of the box C/D family function in directing site-specific 2'-O-methylation of substrate RNAs [cite journal | last = Galardi | first = S | coauthors = Fatica A, Bachi A, Scaloni A, Presutti C, Bozzoni I | year = 2002 | title = Purified box C/D snoRNPs are able to reproduce site-specific 2'-O-methylation of target RNA in vitro | journal = Mol Cell Biol | volume = 22 | pages = 6663–6668 | pmid = 12215523 | doi = 10.1128/MCB.22.19.6663-6668.2002] .

U95 was identified by computational screening of the introns of ribosomal protein genes for conserved C/D box sequence motifs and expression experimentally verfied by northern blotting cite journal | author = Vitali P, Royo H, Seitz H, Bachellerie JP, Hüttenhofer A, Cavaillé J | title = Identification of 13 novel human modification guide RNAs | journal = Nucleic Acids Res. | volume = 31 | issue = 22 | pages = 6543–51 | year = 2003 | pmid = 14602913 | doi = | issn = ] . U95 is predicted to guide the 2'O-ribose methylation of 28S ribosomal RNA (rRNA) residues A2802 and C2811 cite journal | last = Lestrade | first = L | coauthors = Weber MJ | year = 2006 | title = snoRNA-LBME-db, a comprehensive database of human H/ACA and C/D box snoRNAs | journal = Nucleic Acids Res. | volume = 34 | pages = D158–D162 | pmid = 16381836 | doi = 10.1093/nar/gkj002 ] .

References

External links

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* [http://www-snorna.biotoul.fr/plus.php?id=U95 snoRNABase entry for SNORD95]