Insertional inactivation

Insertional inactivation

Insertional inactivation is a technique used in recombinant DNA engineering where a plasmid (such as pBR322) [ [http://www.uark.edu/campus-resources/mivey/m4233/recdna.html Recombinant DNA ] ] is used to disable expression of a gene. [ [http://www.cartage.org.lb/en/themes/Reference/dictionary/Biologie/I/125.html Insertional inactivation ] ]

The inactivation of a gene by inserting a fragment of DNA into the middle of its coding sequence. Any future products from the inactivated gene will not work because of the extra codes added to it.

References

External links

* [http://content.gene-technology-online.com/genlib/selection.htm Diagram at gene-technology-online.com]


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  • insertional inactivation — insertional inactivation. См. инактивация вставкой. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • Insertional mutagenesis — is mutagenesis of DNA by the insertion of one or more bases.[1] Insertional mutations can occur naturally, mediated by virus or transposon, or can be artificially created for research purposes in the lab. Contents 1 Signature tagged mutagenesis 2 …   Wikipedia

  • inactivation — The process of destroying or removing the activity or the effects of an agent or substance; e.g., the complementary effect of a serum may be destroyed by means of i. at 56°C for 30 min. insertional i. a technique of recombinant DNA technology… …   Medical dictionary

  • инактивация вставкой — insertional inactivation инактивация вставкой. Прекращение экспрессии гена в результате вставки <insertion>; метод И.в. используется для выявления рекомбинантной ДНК при клонировании в плазмидных векторах по прекращению экспрессии… …   Молекулярная биология и генетика. Толковый словарь.

  • Recombinant DNA — (rDNA) molecules are DNA sequences that result from the use of laboratory methods (molecular cloning) to bring together genetic material from multiple sources, creating sequences that would not otherwise be found in biological organisms.… …   Wikipedia

  • Molecular cloning — refers to a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms.[1] The use of the word cloning refers to the fact that the method involves the …   Wikipedia

  • Cellular differentiation — Cell differentiation redirects here. For the journal, see Cell Differentiation (journal). In developmental biology, cellular differentiation is the process by which a less specialized cell becomes a more specialized cell type. Differentiation… …   Wikipedia

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  • инактивация вставкой — Прекращение экспрессии гена в результате вставки; метод И.в. используется для выявления рекомбинантной ДНК при клонировании в плазмидных векторах по прекращению экспрессии селектируемого маркера. [Арефьев В.А., Лисовенко Л.А. Англо русский… …   Справочник технического переводчика

  • Инсерционная инактивация — * інсерцыйная інактывацыя * insertional inactivation нарушение кодирующей области гена путем инсерции (включения, вставки) чужеродной ДНК, ведущее к потере функции гена, т. е. к прекращению его экспрессии. И. и. позволяет легко отбирать… …   Генетика. Энциклопедический словарь

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