Nitrite test

A nitrite test is a chemical test used to determine the presence of nitrite ion in solution.

A common nitrite test can be performed by adding 4 M sulfuric acid to the sample until acidic, and then adding 0.1 M iron(II) sulfate to the solution. A positive test for nitrite is indicated by a dark brown solution, arising from the iron-nitric oxide complex ion.^[1]

This test is also commonly used in diagnosing urinary tract infections. A positive nitrite test indicates that the cause of the UTI is a gram negative organism. The reason for nitrites existence in the presence of a UTI is due to a bacterial conversion of endogenous nitrates to nitrites.

See also

• Nitrate test: similar, but uses concentrated sulfuric acid to test for nitrate ion.

References

1. ^ Holltzclaw, H.; Robinson, W. (1988), "College Chemistry with qualitative analysis", Edition, D. C. Heath and Company:Lexington, MA 8: 1006 

This article about analytical chemistry is a stub. You can help Wikipedia by expanding it.v · Categories: Chemical tests Analytical chemistry stubs Wikimedia Foundation. 2010. Игры ⚽ Поможем решить контрольную работу China National Nuclear Corporation Chihiro Suzuki Look at other dictionaries: nitrite test — 1. (for nitrites in saliva) to the saliva add 1 or 2 drops of sulfuric acid, a few drops of potassium iodide solution, and some starch paste; a blue color indicates nitrites. 2. a test for nitrites in any fluid; see specific tests, including… …   Medical dictionary test — 1. To prove; to try a substance; to determine the chemical nature of a substance by means of reagents. 2. A method of examination, as to determine the presence or absence of a definite disease or of some substance in any of the fluids, tissues,… …   Medical dictionary Nitrate test — For the test to determine the ability of microbes to reduce nitrate to nitrite, see Nitrate reductase test. A nitrate test is a chemical test used to determine the presence of nitrate ion in solution. Testing for the presence of nitrate via wet… …   Wikipedia nitrate reduction test — (for bacteriuria or presence of bacteria in other fluids) a type of nitrite test that identifies the reduction of nitrate to nitrite by a bacterial culture. The fluid under investigation is cultured in a broth containing nitrate and the medium is …   Medical dictionary Griess test — The Griess test is a chemical analysis test which detects the presence of organic nitrite compounds. The Griess diazotization reaction on which the Griess reagent relies was first described in 1858 by Peter Griess. MethodNitrite is detected and… …   Wikipedia Nitrate reductase test — The nitrate reductase test is a test to differentiate between bacteria based on their ability or inability to reduce nitrate (NO3−) to nitrite (NO2−) using anaerobic respiration. Procedure Various assays for detecting nitrate reduction have been… …   Wikipedia Drug test — For other uses, see Drug testing (disambiguation). Drug test Diagnostics To minimize opportunities for tampering, a direct line of sight between the observer and the specimen bottle must be maintained during collection of a urine sample …   Wikipedia Chemical test — In chemistry, a chemical test is a qualitative or quantitative procedure designed to prove the existence of, or to quantify, a chemical compound or chemical group with the aid of a specific reagent. A presumptive test is specifically used in… …   Wikipedia van den Bergh's test — a test to determine whether excess bilirubin in the blood is conjugated or unconjugated, and therefore whether jaundice in a patient is due to haemolysis or to disease of the liver or bile duct. A sample of serum is mixed with sulphanilic acid,… …   Medical dictionary Griess test — 1. (for nitrate reducing bacteria in a fluid) a formerly common test in which sulfuric acid and an indicator were added to a dilute solution and color change was assessed. 2. the same name is now applied to nitrite tests for bacteriuria in which… …   Medical dictionary 18+ © Academic, 2000-2024 Contact us: Technical Support, Advertising Dictionaries export, created on PHP, Joomla, Drupal, WordPress, MODx. Mark and share Search through all dictionaries Translate… Search Internet Share the article and excerpts Direct link … Do a right-click on the link above and select “Copy Link”