Denaturing gel

Denaturing gel

A denaturing gel is a type of electrophoresis in which the native structure of macromolecules that are run within the gel is not maintained. For instance, gels used in SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) will unfold and denature the native structure of a protein. In contrast to native gel electrophoresis, quaternary structure cannot be investigated using this method.


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  • Gel electrophoresis — apparatus – An agarose gel is placed in this buffer filled box and electrical field is applied via the power supply to the rear. The negative terminal is at the far end (black wire), so DNA migrates toward the camera. Classification… …   Wikipedia

  • denaturing gradient gel electrophoresis — denaturing gradient gel electrophoresis. См. электрофорез в градиенте денатурирующего геля. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • Denaturing Gradient Gel Electrophoresis — Électrophorèse sur gel en gradient dénaturant L électrophorèse sur gel en gradient dénaturant ou DGGE (acronyme de l anglais denaturing gradient gel electrophoresis) est une technique d électrophorèse permettant la séparation de molécules d… …   Wikipédia en Français

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  • denaturing gradient gel electrophoresis — Abbreviation: DGGE An electrophoresis method for separating similar sized DNA fragments on the basis of their sequence, by applying across the gel a gradient of increasingly denaturing conditions (usually by increasing the concentration of a… …   Glossary of Biotechnology

  • Temperature gradient gel electrophoresis — (TGGE) and Denaturing Gradient Gel Electrophoresis (DGGE) are forms of electrophoresis which use either a temperature or chemical gradient to denature the sample as it moves across an acrylamide gel. TGGE and DGGE can be applied to nucleic acids… …   Wikipedia

  • Electroforesis en gel con gradiente de desnaturalización — Saltar a navegación, búsqueda La electroforesis en gel con gradiente de desnaturalización, también referida como electroforesis en gel desnaturalizante en gradiente, (en el ámbito científico comúnmente referida por su abreviación en inglés: DGGE) …   Wikipedia Español

  • In-gel digestion — The in gel digestion is part of the sample preparation for the mass spectrometric identification of proteins in course of proteomic analysis. The method was introduced 1992 by Rosenfeld.Rosenfeld, J et al, Anal Biochem , 1992, 203 (1), 173 9.]… …   Wikipedia

  • Two-dimensional gel electrophoresis — Two dimensional gel electrophoresis, abbreviated as 2 DE or 2 D electrophoresis, is a form of gel electrophoresis commonly used to analyze proteins. Mixtures of proteins are separated by two properties in two dimensions on 2D gels.Basis for… …   Wikipedia

  • Native Gel Electrophoresis — is a technique used mainly in protein electrophoresis where the proteins are not denatured and therefore separated based on their charge to mass ratio.The two main types of native gels used in protein electrophoresis are polyacrlylamide gels and… …   Wikipedia

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