DHR2 domain

DHR2 (DOCK homology region 2), also known as CZH2 or Docker2, is a protein domain of approximately 450-550 amino acids that is present in the DOCK family of proteins.^[1] This domain functions as a guanine nucleotide exchange factor (GEF) domain for small G proteins of the Rho family.^[2] DHR2 domains bear no significant similarity to the well described DH domain (Dbl homologous domain) present in other RhoGEFs such as Vav, P-Rex and TRIO. Indeed, the most divergent mammalian DHR2 domains share only 16-17% sequence similarity.

References

1. ^ Meller N, Merlot S, Guda C (November 2005). "CZH proteins: a new family of Rho-GEFs". J. Cell Sci. 118 (Pt 21): 4937–46. doi:10.1242/jcs.02671. PMID 16254241. 
2. ^ Côté JF, Vuori K (December 2002). "Identification of an evolutionarily conserved superfamily of DOCK180-related proteins with guanine nucleotide exchange activity". J. Cell Sci. 115 (Pt 24): 4901–13. doi:10.1242/jcs.00219. PMID 12432077. 

Further reading

• Côté JF, Vuori K (2007). "GEF what? Dock180 and related proteins help Rac to polarize cells in new ways". Trends Cell Biol. 17 (8): 383–393. doi:10.1016/j.tcb.2007.05.001. PMC 2887429. PMID 17765544. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2887429. 
• Côté JF, Vuori K (2006). "In vitro guanine nucleotide exchange activity of DHR-2/DOCKER/CZH2 domains". Meth. Enzym. 406: 41–57. doi:10.1016/S0076-6879(06)06004-6. PMID 16472648. 
• Lu M, Kinchen JM, Rossman KL, et al. (2005). "GEF A Steric-inhibition model for regulation of nucleotide exchange via the Dock180 family of GEFs". Curr. Biol. 15 (4): 371–77. doi:10.1016/j.cub.2005.01.050. PMID 15723800.