RIPA buffer

RIPA buffer

RIPA buffer is a lysis buffer used to lyse cells and tissue, for radio immunoprecipitation assay (RIPA).

RIPA buffer recipe for total protein extract is as follows:

* Tris 50mM
* NaCl 150 mM
* SDS 0.1 %
* Na.Deoxycholate 0.5 %
* Triton X 100 or NP40 1%
* Protease inhibitors such as PMSF or cocktail protease inhibitors which are commercially available (use according to the manufacturer's guide)

External links

* [http://www.piercenet.com/Objects/View.cfm?type=ProductFamily&id=9984522F-D74A-4E46-829C-C057D8C21050]
* [http://www.cellsignal.com/products/9806.html]
* [http://carmen.salk.edu/~sefton/Hyper_protocols/immunoprecip.html#anchor1446740]
* [http://www.molecularstation.com/protein/immunoprecipitation/]


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